Insulate Britain has blocked two sections of major roads into London after warning its "non-violent civil resistance" would continue on Wednesday.
The road-blocking climate group obstructed the A40 in west London and a roundabout in Dartford in the east of the capital to urge the Government to better insulate homes.
One member said she was prepared to lose her home and go to jail for the cause.
Suzie, 47, from Cambridge, who works in childcare, said: "If going to prison and losing my home is what it takes to get the Government to do the right thing and cut our carbon emissions then it's a price worth paying.
"I can't be a bystander while this Government betrays the public, our children and future generations by failing to defend our country from the climate crisis.
"Insulating Britain's leaky homes has to come first. It will cut out a huge chunk of our carbon emissions, help families struggling with their energy bills and end fuel poverty.
"It's such a basic first step that you have to wonder why the Government refuses to act. Don't they care?"
The latest action comes despite four court injunctions taken out in response to Insulate Britain protests.
Most recently the Government-owned National Highways secured a ban on activities which obstruct traffic on its 4,300-mile network of motorways and major A-roads in England.
This came in the wake of a super injunction granted to National Highways on Monday, which prohibits people from Insulate Britain from interfering with traffic on any part of the strategic road network in England.
Videos from the protests showed police having to separate angry motorists attempting to remonstrate with protesters.
One man could be heard saying: "We're trying to earn a living here and some people are trying to get their kids to school. Move."