The actor had played Frankie Lewis in the BBC soap since 2020 and was the show's first regular deaf cast member.
But last month she announced that it was time to move on, saying that although she would always "treasure" her time in Albert Square, she was "excited and ready for new challenges".
The soap tweeted a goodbye to the star before her final episode, saying how much she'd be missed.
"Tonight we say goodbye to Frankie Lewis and the gorgeous @RoseAylingEllis!" they said.
"Walford won't be the same without you."
Ayling-Ellis retweeted in and wrote: "Thank you for all the love and support.
"I really appreciate it."
Thank you for all the love and support. I really appreciate it 💕 https://t.co/53lSOJ7m6e
— Rose Ayling-Ellis (@RoseAylingEllis) September 23, 2022
She was in the competition last year, becoming the first ever deaf contestant to take part in the BBC ballroom contest.
During her time on the show she won praise for a routine that she danced partly in silence to show what her experience of the show was like.
Camilla, Queen Consort, said at an EastEnders set visit: "Rose, the Strictly star. I said we were all voting for Rose, I don't know how you did all those dances."
Ayling-Ellis then said: "Can I ask you, does the Queen watch Strictly Come Dancing?"
Camilla replied: "I think she does. She probably watched you."
Watch: Rose Ayling-Ellis denies rumours she's set to star in Doctor Who